Automation of Fire Monkey HMW-­DNA extraction in multi-­well filter plate format

We have configured and validated a bespoke, fully-automated workflow that integrates an adapted Tecan Fluent 780 robotic liquid handler with an integrated Rotanda 460 robotic centrifuge. Our patented Fire Monkey chemistry and matrix in a laboratory-standard multi-well plate kit enables automation of the Fire Monkey spin-based process.

Working with our automation partner, Tecan, the fully-automated workflow integrates an adapted Tecan Fluent 780 robotic liquid handler with an integrated Rotanda 460 robotic centrifuge using laboratory standard multi-well plates, with patented Fire Monkey chemistry and matrix, enabling automation of our spin-based process.

Automation of DNA extraction, the critical first step in the DNA sequencing process, comes at a good time as structural variant surveillance could drive a sequencing boom. The push to keep COVID-19 variants at bay could be the point that turns genomic sequencing from a sophisticated technique into a widely accessible genomic tool, withleading companies focused on automation.

Biocentury, 27 February 2021

Validation

Our new automated Fire Monkey HMW-DNA extraction protocol has been validated to extract and purify bacterial samples.

In this validation run to demonstrate quality of sequencing output, eight E. coli cell suspensions were transferred to a 96-well plate and, after a fully hand-free process, the output DNA delivered sequencing results equivalent to manually-extracted Fire Monkey spin-column based HMW-DNA. The average strand length achieved was 106kb with sequencing on the Oxford Nanopore (ONT) MinION LSK110 platform delivering more than 30Gb at N50 longer than 30kb, with good pore health (Figure 1).

Figure 1: Automated Fire Monkey HMW-DNA extraction filter-plate protocol on Tecan Fluent 780 robotic liquid handling platform, validated by sequencing
08 Oct, 2020 ITN Productions

Dr Gemma Langridge, Group Leader, Quadram Institute, comments in the ‘Biology Impacting Lives’ programme from ITN Productions in partnership with the Royal Society of Biology

With the automation that is coming from RevoluGen, what we are looking for is to be able to prep hundreds of bacterial strains at a time, then the scope for our research increases quite dramatically that way

Fire Monkey automation

Further optimization of the multi-well filter plates is ongoing in RevoluGen’s R&D laboratories with commercial launch of the automated HMW-DNA extraction kit anticipate in the coming months.

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Research & Manufacturing site:

RevoluGen Ltd
Unit 4 Rossington Place
Graphite Way
Hadfield, Derbyshire
SK13 1QG, UK

VAT No. GB 712 2187 67

Head Office:

RevoluGen Ltd
32­-33 High Street
Ascot, Berkshire
SL5 7HG, UK

Registered company No. 03518866
Registered in England and Wales

Follow:
Proud members of:
bia reach ready
bionow
Research & Manufacturing site:

RevoluGen Ltd
Unit 4 Rossington Place
Graphite Way
Hadfield, Derbyshire
SK13 1QG, UK

VAT No. GB 712 2187 67

Head Office:

RevoluGen Ltd
32­-33 High Street
Ascot, Berkshire
SL5 7HG, UK

Registered company No. 03518866
Registered in England and Wales