Fire FlowerFire Flower selection protocol that removes the shorter DNA fragments from extracted samples
Fire Flower is a user friendly spin column protocol with a built in size depletion ability included in the Fire Monkey kit, and can be used with any other extraction protocol.
Fire Flower can be used to size select up to 200µl of extracted DNA despite the sample being rich in small DNA fragments. The protocol takes just 15mins resulting in an extract which will effectively double most long-read metrics on the LSK109/MinION technology.
Other key features:
- The Fire Flower size selection protocol is included in the Fire Monkey kit.
- Fire Flower improves the ratio of high molecular weight versus low molecular weight DNA.
- Can increase the overall average DNA strand length by 30%.
- Broad applicability and agnostic of sequencing process used.
- Can be used with any other extraction protocol.
- Can be used to size select up to 200µl of extracted DNA despite the sample being rich in small DNA fragments.
10 columns Kit
50 columns Kit
For Research Use Only. Not intended for diagnostic purposes.
For Research Use Only. Not intended for diagnostic purposes.
£195.00 Plus VAT
via distributors
Fire Flower
RevoluGen’s Fire Flower protocol by itself can be used independently on any extraction technology and can potentially double the long-read sequencing metrics as shown below
Product Information
Instructions for use for 10 Column Kit
(PDF)
Instructions for use for 50 Column Kit
(PDF)
Safety Data Sheets
Lysis Solution
(PDF)
Binding Solution
(PDF)
Wash Solution
(PDF)
Elution Buffer
(PDF)
