Fire Flower selection protocol that removes the shorter DNA fragments from extracted samples

Fire Flower is a user­ friendly spin­ column protocol with a built ­in size depletion ability included in the Fire Monkey kit, and can be used with any other extraction protocol.

Fire Flower can be used to size­ select up to 200µl of extracted DNA despite the sample being rich in small DNA fragments. The protocol takes just 15mins resulting in an extract which will effectively double most long­-read metrics on the LSK109/MinION technology.

Other key features:

  • The Fire Flower size selection protocol is included in the Fire Monkey kit.
  • Fire Flower improves the ratio of high molecular weight versus low molecular weight DNA.
  • Can increase the overall average DNA strand length by 30%.
  • Broad applicability and agnostic of sequencing process used.
  • Can be used with any other extraction protocol.
  • Can be used to size­ select up to 200µl of extracted DNA despite the sample being rich in small DNA fragments.

10 columns Kit

50 columns Kit

For Research Use Only. Not intended for diagnostic purposes.

For Research Use Only. Not intended for diagnostic purposes.

FIRE MONKEY & FIRE FLOWER 10 COLUMNS KIT
£195.00 Plus VAT
FIRE MONKEY & FIRE FLOWER 50 COLUMNS KIT
via distributors

Fire Flower

1

RevoluGen’s Fire Flower protocol by itself can be used independently on any extraction technology and can potentially double the long-read sequencing metrics as shown below


FF entry 1

Product Information

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Instructions for use for 10 Column Kit
(PDF)

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Instructions for use for 50 Column Kit
(PDF)

Safety Data Sheets

pdf_download

Lysis Solution
(PDF)

pdf_download

Binding Solution
(PDF)

pdf_download

Wash Solution
(PDF)

pdf_download

Elution Buffer
(PDF)

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Research & Manufacturing site:

RevoluGen Ltd
Unit 4 Rossington Place
Graphite Way
Hadfield, Derbyshire
SK13 1QG, UK

VAT No. GB 712 2187 67

Head Office:

RevoluGen Ltd
32­-33 High Street
Ascot, Berkshire
SL5 7HG, UK

Registered company No. 03518866
Registered in England and Wales

Follow:
Proud members of:
bia reach ready
Research & Manufacturing site:

RevoluGen Ltd
Unit 4 Rossington Place
Graphite Way
Hadfield, Derbyshire
SK13 1QG, UK

VAT No. GB 712 2187 67

Head Office:

RevoluGen Ltd
32­-33 High Street
Ascot, Berkshire
SL5 7HG, UK

Registered company No. 03518866
Registered in England and Wales