RevoluGen - DNA amplification technolgy

 

 Purispin

Purispin is a centrifugal based nucleic acid isolation and purification (NAIP) process that utilises a proprietary mixture of original chemistry and materials to deliver what is effectively a “more gentle” rapid nucleic acid extraction process. The key benefit of the Purispin technology is that it extracts high quality and very long DNA fragments with many fragments of between 165,000 and 240,000 base pairs long in under one hour.

The following graphic illustrates the outline process 

Revolugen NAIP kits Fire Monkey

The Purispin extraction protocols for white blood cells, mammalian cells and bacteria have been optimised and protocols for the extraction from solid tissue FFPE, plants and whole blood are being developed. The extraction protocol can be tuned so as to deliver either pure DNA, pure RNA or total nucleic acid extracts.

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This agarose gel electrophoresis picture above, demonstrates Purispin technology extracting DNA from Horse Blood white cells. This extracted DNA shows intact DNA fragments with lengths of >60,000 base pairs; which was about the limit of the range for this detection system. The High DNA Integrity Number (DIN) consistently achieved on these multiple extraction runs compared to the competitors is shown by the reduced smears under the bulk line for the long DNA.

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This agarose gel electrophoresis picture above, demonstrates Purispin technology results from extracting total nucleic acid from the HeLa mammalian cell line. The total DNA and RNA extracted is of excellent quality and quantity which is useful for precious samples.

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This agarose gel electrophoresis picture above, demonstrates Purispin technology results from extracting pure RNA from bacterial cells. There is minimal DNA contamination and excellent purity. This was all accomplished in less than one hour and without the use of any toxic chemicals such as ß-mercaptoethanol.

RevoluGen-Purispin-DNA-Diagram-Feb-18-updates

This graphic above, shows the DIN comparison figures for Purispin, as produced by Dundee University, compared to a major competitor in this group of centrifugal based NAIP kits that take around an hour to perform. The picture is a scanning electron micrograph picture of the stained DNA extracted by Purispin. This picture was taken by Warwick University using their proprietary staining technique which illustrates the unravelled DNA molecule and shows why the gel electrophoresis techniques have difficulty separating out very long DNA molecules.

RevoluGen-DNA-Femtopulse-Jan-18

This graph above was produced by a femto-pulse machine which is able to differentiate the very long DNA fragments much more clearly. The X axis shows the molecule lengths in base pairs whilst the Y axis shows the mass of the molecules of that particular length. In this run the Purispin technology has delivered a significant number of molecules with lengths above 165,000 base pairs and even peaks at around the 240,000 base pair point.

Revolugen NAaIP technology long chain DNA

This picture is a summary of the main competitive points in favour of the Purispin technology over the existing technologies. Purispin is fast to perform at under one hour which is much the same as other centrifugal NAIP kits even whilst it is delivering much longer intact DNA fragments than the competition. Purispin protocols yield DNA fragment length of over 165,000 base pairs which is just what evolving techniques such as long read sequencing and long range PCR require. High purity, quality and flexibility of outcome as well as lack of toxicity and fewer steps make Purispin technology potentially disruptive.