NEW YORK (GenomeWeb) – As long-read sequencing and genome mapping technologies from companies like Pacific Biosciences, Oxford Nanopore Technologies, Bionano Genomics, and 10x Genomics are gaining in popularity, a bottleneck has emerged around the ability to quickly and efficiently extract high-quality high molecular weight DNA.

Several companies have taken on the challenge, including Circulomics, Bionano, Sage Science, and RevoluGen, with the goal of developing high-throughput methods for extracting DNA hundreds of kilobases and up to megabases in length from different sample types. In addition, academic groups have been developing their own protocols or optimizing commercial ones to meet their specific needs.

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Long Read DNA Extraction